Gene Transfer and Expression in Mammalian Cells

Front Cover
S.C. Makrides
Gulf Professional Publishing, Nov 7, 2003 - Science - 680 pages
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.

This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.
 

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Contents

Stable gene expression
14
Using HSV1 to make gene therapy vectors
33
Applications of HSV vectors in the nervous system
42
Applications outside the nervous system
48
Cellspecific gene expression
64
Abbreviations
89
Production of recombinant AAV vectors
97
In vitro and in vivo gene transfer
99
Conclusions
165
Poliovirus vector development and its immunogenic potential
176
Abbreviations
185
Insect and crustacea
195
Abbreviations
203
Expression of topologically different proteins
209
Scaleup of protein production
215
Expression in primary cell cultures
216

Abbreviations
105
Gene delivery
115
Production and analyses of viral particles
121
Construction of recombinant poxvirus vectors
127
Laboratory and clinical applications
132
Molecular biology of virus vector construction
139
Applications of the baculovirus mammalian gene delivery vector
145
Abbreviations
226
Development of recombinant retrovirus vectors
235
Expression of the transgene
242
References
248
HIV1derived vector packaging system
255
Gene transfer applications
262
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