Gene Transfer and Expression in Mammalian CellsS.C. Makrides The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field. |
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Abbreviations | 6 |
6 | 21 |
Using HSV1 to make gene therapy vectors | 33 |
Copyright | |
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AAV vectors Acad acid activity adenovirus antibody antigen apoptosis baculovirus binding Biol Biotechnol capsid caspases cDNA cell lines cell types cellular Chem chromatin chromosomal clones codon complex containing copy number cytoplasm degradation deleted domain efficient elements encoding enhancer enzymes episomal expression levels expression vectors factor function fusion gene delivery gene expression Gene Ther gene therapy gene transfer genetic genome glycans glycoprotein heterologous host cell HSV vectors human immune response infection inhibition integration interaction intrabody mammalian cells membrane molecules mRNA mutant Natl neurons nuclear oriP packaging particles pathway plasmid poliovirus Proc production promoter proteasome receptor recombinant proteins region regulation regulatory replication replicon reporter gene ribosome selection sequences SFV vectors signal specific stable strategy structure substrate target tissue transcription transduction transfection transgene transgene expression translation tumor ubiquitin vaccine viral genome virion Virol virus viruses vitro vivo